Phytochemical Qualitative Analysis and Immunomodulator Activity of Agaricus bisporous Ethanol Extract by Carbon Clearance Technique

The Agaricus bisporous have been collected and its extracts are subjected to qualitative analysis and immunomodulator activity. Quantitative analysis has been carried out to find some phytochemicals presents in the sample. These Ethanol extracts revealed with the presence of the constituents such as Sterols, Saponins, Coumarin Glycosides, Amino acid/ protein, Resins. A preliminary phytochemical screening has been conducted with the selected fungal extracts by using standard C.K.Kokate procedures and it is revealed that several secondary metabolites are present in the sample. In vivo the effect of ethanol extract of the two Agaricus bisporous has been applied on neutrophil phagocytosis activity. Various concentrations namely 50, 100 and 200 μg/ml of the ethanol extract has been tested for phagocytosis as neutrophil locomotion. This has been tested with Swiss albino mice with selected dose of ethanol concentrations in 50, 100 and 200 mg/kg body weight has been administrated in order to confirm this with macrophage phagocytosis activity by carbon clearance test. In vivo studies of Agaricus bisporous revels the moderate immune stimulator activity, thus this fungal species have demonstrated the prominent immunostimulator activity. The data generated in this study has the basics for its use as the therapeutic both as traditional and folk medicine as a widely as to be practiced. Phytochemical Qualitative Analysis and Immunomodulator Activity of Agaricus bisporous Ethanol Extract by Carbon Clearance Technique